| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wood, Ashley J (14)
|
MDHFY |
59.09 |
1:02.68 |
3.59 |
| 2 |
Smith, Kayla M (14)
|
MDTCY |
|
1:03.68 |
- |
| 3 |
Westerfield, Isabella G (14)
|
MDTCY |
|
1:03.98 |
- |
| 4 |
Hull, Madeline A (14)
|
MDTCY |
|
1:06.70 |
- |
| 5 |
Price, Isabella (14)
|
MDHFY |
1:06.90 |
1:07.21 |
0.31 |
| 6 |
Robertson, Mariah C (14)
|
MDHFY |
1:05.25 |
1:09.17 |
3.92 |
| 7 |
Morris, Claire E (13)
|
MDTCY |
|
1:09.67 |
- |
| 8 |
Sheikh, Aaliyah A (14)
|
CECY |
1:10.15 |
1:10.13 |
-0.02 |
| 9 |
Dickerson, Marissa A (13)
|
MDHFY |
1:13.37 |
1:10.46 |
-2.91 |
| 10 |
Mareck, Avery (13)
|
MDHFY |
1:19.77 |
1:18.96 |
-0.81 |
| 11 |
Ladd, Leah A (13)
|
MDHFY |
1:17.56 |
1:20.90 |
3.34 |