| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Morales, Erick (15)
|
NTCOR |
55.82 |
56.77 |
0.95 |
| 2 |
Roman, Andrew C (15)
|
NTSTAR |
58.25 |
57.84 |
-0.41 |
| 3 |
Roa-Irizarry, Marlon D (17)
|
NTCOPS |
59.94 |
57.88 |
-2.06 |
| 4 |
Feldhousen, Alec A (19)
|
NTRACE |
56.10 |
58.49 |
2.39 |
| 5 |
Clark, Soren M (15)
|
NTSTAR |
1:01.62 |
58.63 |
-2.99 |
| 6 |
Sutton, Caelan G (16)
|
NTRACE |
58.41 |
58.99 |
0.58 |
| 7 |
Wigand, Dane A (15)
|
NTSTAR |
58.23 |
59.23 |
1.00 |
| 8 |
Bleggi, Pasquale A (17)
|
NTSTAR |
1:01.03 |
1:00.07 |
-0.96 |
| 9 |
Patterson, Tyler R (15)
|
NTESP |
58.28 |
1:00.28 |
2.00 |
| 10 |
Tu, Nicholas W (15)
|
NTCOR |
1:00.20 |
1:01.08 |
0.88 |
| 11 |
Liu, Hao Ran (15)
|
NTCOPS |
1:01.53 |
1:02.20 |
0.67 |
| 12 |
Huffman, Luke K (16)
|
NTSTAR |
1:01.05 |
1:03.09 |
2.04 |
| 13 |
Lawshe, Isaiah L (15)
|
NTSTAR |
1:03.67 |
1:03.59 |
-0.08 |
|
Campbell, Alexander C (15)
|
NTCOR |
58.68 |
NS |
- |
|
Knowles, Sebastian A (16)
|
NTSTAR |
57.34 |
NS |
- |
|
Vedala, Avyay S (15)
|
NTSTAR |
57.20 |
NS |
- |