| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dobrott, John R (15)
|
NTCOPS |
24.96 |
25.22 |
0.26 |
| 2 |
Sutton, Caelan G (16)
|
NTRACE |
25.93 |
25.61 |
-0.32 |
| 3 |
Roman, Andrew C (15)
|
NTSTAR |
26.10 |
25.66 |
-0.44 |
| 4 |
Wigand, Dane A (15)
|
NTSTAR |
25.99 |
26.07 |
0.08 |
| 5 |
Bleggi, Pasquale A (17)
|
NTSTAR |
26.75 |
26.73 |
-0.02 |
| 6 |
Roa-Irizarry, Marlon D (17)
|
NTCOPS |
27.17 |
27.33 |
0.16 |
| 7 |
Patterson, Tyler R (15)
|
NTESP |
26.88 |
27.45 |
0.57 |
| 8 |
Huffman, Luke K (16)
|
NTSTAR |
26.64 |
27.62 |
0.98 |
| 9 |
Clark, Soren M (15)
|
NTSTAR |
27.40 |
27.68 |
0.28 |
| 10 |
Tu, Nicholas W (15)
|
NTCOR |
28.13 |
28.20 |
0.07 |
| 11 |
Liu, Hao Ran (15)
|
NTCOPS |
27.85 |
28.83 |
0.98 |
| 12 |
Lawshe, Isaiah L (15)
|
NTSTAR |
29.03 |
29.03 |
0.00 |
|
Campbell, Alexander C (15)
|
NTCOR |
27.09 |
NS |
- |
|
Knowles, Sebastian A (16)
|
NTSTAR |
25.10 |
NS |
- |
|
Vedala, Avyay S (15)
|
NTSTAR |
25.69 |
NS |
- |