| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smetana, Shelby T (7)
|
NTNTN |
37.31 |
35.35 |
-1.96 |
| 2 |
Wilson, Elyse H (8)
|
NTJMSS |
40.63 |
39.44 |
-1.19 |
| 3 |
Wright, Ava L (8)
|
NTMTRO |
40.23 |
40.34 |
0.11 |
| 4 |
Ossai, Chinasa N (8)
|
NTLTSD |
38.75 |
42.31 |
3.56 |
| 5 |
Lazaro, Victoria E (8)
|
NTATAC |
42.02 |
43.51 |
1.49 |
| 6 |
Gamage, Sasmi N (8)
|
NTMTRO |
45.35 |
43.59 |
-1.76 |
| 7 |
Holladay, Hallie A (6)
|
NTATAC |
44.77 |
43.99 |
-0.78 |
| 8 |
McGuinness, Avery A (8)
|
NTLTSD |
46.16 |
45.03 |
-1.13 |
| 9 |
Persefield, Abigail E (8)
|
NTATAC |
43.77 |
47.60 |
3.83 |
| 10 |
Barrett, Leah A (8)
|
NTNTN |
45.50 |
47.66 |
2.16 |
| 11 |
Dobysh, Sofia (8)
|
NTESP |
52.29 |
50.92 |
-1.37 |
| 12 |
Marshall, Hailey G (8)
|
NTESP |
46.57 |
51.22 |
4.65 |
| 13 |
Wilson, Kaitlyn M (7)
|
NTSTAR |
50.64 |
53.55 |
2.91 |
| 14 |
McElrath, Leah M (8)
|
NTLTSD |
|
54.53 |
- |
| 15 |
Kaden, Kinsler C (7)
|
NTATAC |
53.78 |
56.92 |
3.14 |
| 16 |
Fiderfish, Elizabeth V (8)
|
NTLTSD |
|
59.25 |
- |
|
Xanthos, Jordan (7)
|
NTMTRO |
45.42 |
NS |
- |
|
Gonzalez, Erin C (8)
|
NTMTRO |
41.22 |
NS |
- |
|
Vijey, Hira (8)
|
NTUN |
49.23 |
NS |
- |