| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Roundtree, Charis T (10)
|
PVRIPS |
35.21 |
36.88 |
1.67 |
| 2 |
Thaivalappil, Maya E (10)
|
PVPM |
|
39.54 |
- |
| 3 |
McDonald, Mia S (10)
|
PVPM |
|
40.67 |
- |
| 4 |
Masten, Payton E (10)
|
PVSNOW |
39.47 |
41.16 |
1.69 |
| 5 |
Chen, Sunny B (10)
|
PVSNOW |
41.33 |
41.31 |
-0.02 |
| 6 |
Carreon, Isabella D (9)
|
PVUN |
41.31 |
41.73 |
0.42 |
| 7 |
Nguyen, Lien J (10)
|
PVPM |
42.82 |
42.75 |
-0.07 |
| 8 |
Takach, Alexandra E (10)
|
PVSNOW |
47.52 |
44.21 |
-3.31 |
| 9 |
Killian, Scarlett G (10)
|
PVUN |
45.42 |
45.72 |
0.30 |
| 10 |
Sparrenberger, Riley M (9)
|
PVSNOW |
45.23 |
46.39 |
1.16 |
| 11 |
Sun, Faith N (9)
|
PVUN |
47.07 |
46.99 |
-0.08 |
| 12 |
Bui, Emma T (9)
|
PVMACH |
46.60 |
47.62 |
1.02 |
| 13 |
Saunders, Riley C (9)
|
PVPM |
47.84 |
47.73 |
-0.11 |
| 14 |
Fitzgerald, Tennyson E (10)
|
PVPM |
|
47.87 |
- |
| 15 |
King, Anna M (9)
|
PVMACH |
45.90 |
52.47 |
6.57 |
| 16 |
Wilson, Mara B (7)
|
PVMACH |
52.82 |
52.96 |
0.14 |
| 17 |
Killian, Renee H (9)
|
PVUN |
50.52 |
54.07 |
3.55 |
| 18 |
Sarathy, Anoushka (8)
|
PVMACH |
53.09 |
54.17 |
1.08 |
|
Antognoli, Marley S (10)
|
PVSNOW |
45.37 |
NS |
- |
|
McCarney, Maya R (8)
|
PVSNOW |
50.46 |
NS |
- |
|
Dinh, Lam N (9)
|
PVMACH |
43.27 |
NS |
- |