| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Davies, Cody J (15)
|
MDLBA |
50.06 |
50.06 |
0.00 |
| 2 |
Myers, Benjamin A (18)
|
MDLBA |
48.50 |
51.88 |
3.38 |
| 3 |
Zavrel, Thomas M (17)
|
PVMACH |
52.20 |
52.52 |
0.32 |
| 4 |
Feracci, Dominic V (13)
|
MDLBA |
53.84 |
53.56 |
-0.28 |
| 5 |
Holman, John J (15)
|
MDLBA |
52.08 |
53.67 |
1.59 |
| 6 |
Nealis, Ronan M (18)
|
MDLBA |
52.63 |
53.91 |
1.28 |
| 7 |
Sturgill, Wesley N (15)
|
PVMACH |
52.49 |
53.95 |
1.46 |
| 8 |
Read, Brendan T (16)
|
PVMACH |
55.04 |
54.52 |
-0.52 |
| 9 |
Doyle, Max A (16)
|
PVMACH |
|
55.09 |
- |
| 10 |
Celtnieks, Penelope R (16)
|
MDLBA |
54.07 |
55.14 |
1.07 |
| 11 |
Rosen, Chase J (15)
|
PVMACH |
55.61 |
55.56 |
-0.05 |
| 12 |
Weinstock, Harrison L (14)
|
PVMACH |
57.70 |
56.64 |
-1.06 |
| 13 |
Mueller, Michael J (15)
|
PVMACH |
57.93 |
56.86 |
-1.07 |
| 14 |
Aslaksen, Matthew Y (13)
|
PVMACH |
59.57 |
57.61 |
-1.96 |
| 15 |
Plimpton, Jeremy C (16)
|
MDLBA |
57.10 |
57.70 |
0.60 |
| 16 |
Gilbert, Samantha R (14)
|
PVMACH |
58.44 |
58.14 |
-0.30 |
| 17 |
Reinhardt, Kariana A (15)
|
PVMACH |
57.79 |
59.36 |
1.57 |
| 18 |
Legambi, Jamie T (17)
|
MDLBA |
57.28 |
59.50 |
2.22 |
| 19 |
Egbarin-Aho, Edem E (14)
|
PVMACH |
56.80 |
59.80 |
3.00 |
| 20 |
Argueta, Elena R (14)
|
PVMACH |
59.21 |
1:00.50 |
1.29 |
| 21 |
Bajwa, Elisabeth J (16)
|
PVMACH |
1:02.96 |
1:00.51 |
-2.45 |
| 22 |
Shinnick, Brooke C (16)
|
MDLBA |
55.04 |
1:00.65 |
5.61 |
| 23 |
Johnson, Kennedi T (17)
|
PVMACH |
1:02.98 |
1:02.07 |
-0.91 |
| 24 |
Bennett, Scarlett G (13)
|
PVMACH |
1:04.85 |
1:03.76 |
-1.09 |
| 25 |
Pham, Gabrielle M (13)
|
PVMACH |
|
1:06.98 |
- |
| 26 |
Cobb, Owen C (13)
|
PVMACH |
1:20.81 |
1:16.75 |
-4.06 |
|
Lauretti, Daniel D (15)
|
PVMACH |
1:02.01 |
NS |
- |