| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dinh, Lam N (7)
|
PVMACH |
39.29 |
43.75 |
4.46 |
| 2 |
Walters, Emily J (7)
|
PVFISH |
45.96 |
44.72 |
-1.24 |
| 3 |
Schang, Madison S (7)
|
PVPM |
47.33 |
45.63 |
-1.70 |
| 4 |
Christofferson, Marie N (7)
|
PVMACH |
47.50 |
46.15 |
-1.35 |
| 5 |
Fitzgerald, Tennyson E (7)
|
PVPM |
|
47.68 |
- |
| 6 |
Whitcomb, Kylee C (7)
|
PVPM |
47.04 |
47.96 |
0.92 |
| 7 |
Cushman, McKinley L (7)
|
PVPM |
|
48.18 |
- |
| 8 |
Vasser, Kaidyn A (7)
|
PVNCAP |
|
48.28 |
- |
| 9 |
Barbee, Madeline R (7)
|
PVYORK |
50.54 |
48.76 |
-1.78 |
| 10 |
Benisch, Ela R (7)
|
PVYORK |
|
49.88 |
- |
| 11 |
Jackson, Madison C (7)
|
PVMACH |
51.51 |
51.68 |
0.17 |
| 12 |
Juster, Anne J (7)
|
PVMACH |
|
54.38 |
- |
| 13 |
Beelaert, Anna C (7)
|
PVPM |
|
55.37 |
- |
| 14 |
Nguyen, Alyssa T (7)
|
PVNCAP |
|
55.71 |
- |
| 15 |
Kuzmuk, Campbell R (7)
|
PVYORK |
|
56.49 |
- |
| 16 |
Curry, Haley A (7)
|
PVMACH |
59.03 |
56.59 |
-2.44 |
| 17 |
McMullin, Fay L (7)
|
PVPM |
|
57.51 |
- |
| 18 |
Chow, Chloe S (7)
|
PVMACH |
|
1:00.40 |
- |
| 19 |
Mann-Webb, Lily M (7)
|
PVMACH |
57.88 |
1:06.93 |
9.05 |
| 20 |
Coughlin, Meghan E (7)
|
PVMACH |
|
1:08.23 |
- |
| 21 |
Hu, Kamilla K (7)
|
PVMACH |
1:02.38 |
1:12.59 |
10.21 |
|
Lay, Joset G (7)
|
PVYORK |
1:03.55 |
NS |
- |