| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kovalsky, Donovan R (13)
|
PVNCAP |
26.89 |
26.57 |
-0.32 |
| 2 |
Church, Anderson N (13)
|
PVMACH |
29.15 |
27.71 |
-1.44 |
| 3 |
Manley, Adam J (14)
|
PVNCAP |
29.26 |
27.94 |
-1.32 |
| 4 |
Aslaksen, Luke P (14)
|
PVMACH |
|
27.97 |
- |
| 5 |
Geoghegan, Jake M (14)
|
PVMACH |
|
27.99 |
- |
| 6 |
Rushforth, James T (14)
|
PVMACH |
30.14 |
28.08 |
-2.06 |
| 7 |
Tsuchitani, Jack B (14)
|
PVNCAP |
31.19 |
28.10 |
-3.09 |
| 8 |
Stader, Ryan M (14)
|
PVMACH |
34.45 |
28.25 |
-6.20 |
| 9 |
Golembeski, Joshua A (14)
|
PVMACH |
27.55 |
28.62 |
1.07 |
| 10 |
Sturgill, Wesley N (13)
|
PVMACH |
32.94 |
29.33 |
-3.61 |
| 11 |
Hinkeldey, William R (13)
|
PVMACH |
|
29.38 |
- |
| 12 |
Ong, Marcus Anthony C (14)
|
PVNCAP |
31.09 |
29.50 |
-1.59 |
| 13 |
Philipov, Daniel A (13)
|
PVNCAP |
32.07 |
29.79 |
-2.28 |
| 14 |
Opipari, Nicolas S (13)
|
PVASA |
29.26 |
29.91 |
0.65 |
| 15 |
Ho, Nathan M (14)
|
PVMACH |
34.56 |
29.93 |
-4.63 |
| 16 |
Piccolo, James F (14)
|
PVNCAP |
29.84 |
29.95 |
0.11 |
| 17 |
Bordovsky, Joshua J (13)
|
PVMACH |
31.45 |
30.35 |
-1.10 |
| 18 |
Wolf, Joshua K (13)
|
PVAAC |
|
30.42 |
- |
| 19 |
Klopson, Jack A (14)
|
PVNCAP |
32.64 |
30.63 |
-2.01 |
| 20 |
Lee, Malcolm E (13)
|
PVMACH |
35.15 |
30.81 |
-4.34 |
| 21 |
Wolf, Caleb W (13)
|
PVAAC |
|
30.99 |
- |
| 22 |
Wan, Justin L (13)
|
PVASA |
|
31.35 |
- |
| 23 |
McCrea, Ethan T (13)
|
PVMACH |
33.02 |
31.51 |
-1.51 |
| 24 |
Chua, Daniel S (13)
|
PVMACH |
32.92 |
31.62 |
-1.30 |
| 25 |
Jones, Aidan B (14)
|
PVNCAP |
35.76 |
32.68 |
-3.08 |
| 26 |
Donahue, Caden P (13)
|
PVASA |
32.00 |
33.06 |
1.06 |
| 27 |
Black, Joshua M (13)
|
PVMACH |
|
36.47 |
- |
| 28 |
Ashby, Jordan P (13)
|
PVNCAP |
41.98 |
37.60 |
-4.38 |
|
Bordovsky, Nicholas C (14)
|
PVMACH |
28.03 |
NS |
- |
|
Minsky, Alexander M (13)
|
PVNCAP |
33.02 |
NS |
- |