| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Eliason, Sarah K (11)
|
PVMACH |
29.86 |
29.69 |
-0.17 |
| 2 |
Charters, Hannah M (12)
|
PVMACH |
30.59 |
30.18 |
-0.41 |
| 3 |
Karp, Sienna A (12)
|
PVASA |
33.33 |
31.27 |
-2.06 |
| 4 |
Servaites, Isabel M (12)
|
PVUN-M |
34.89 |
31.63 |
-3.26 |
| 5 |
Morgan, Healey G (11)
|
PVASA |
34.64 |
32.45 |
-2.19 |
| 6 |
Allred, Lilly R (12)
|
PVMACH |
33.02 |
32.92 |
-0.10 |
| 7 |
Shimp, Caroline E (12)
|
PVMACH |
38.84 |
33.70 |
-5.14 |
| 8 |
Rodas, Cheyenne E (12)
|
PVMACH |
34.38 |
33.71 |
-0.67 |
| 9 |
Buswell, Piper M (12)
|
PVNCAP |
34.72 |
34.05 |
-0.67 |
| 10 |
Haryanto, Karolina M (12)
|
PVMACH |
35.50 |
34.89 |
-0.61 |
| 11 |
Lemar, Samantha L (11)
|
PVASA |
|
35.11 |
- |
| 12 |
Gilbert, Samantha R (11)
|
PVCSC |
38.20 |
36.12 |
-2.08 |
| 13 |
Rice, Maren S (11)
|
PVNCAP |
35.44 |
37.54 |
2.10 |
| 14 |
Sandonas, Alexia-Maria P (11)
|
PVASA |
|
45.49 |
- |