| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Li, Vivien Y (13)
|
AMANSC |
2:46.09 |
2:43.96 |
-2.13 |
| 2 |
Khoury, Rita M (14)
|
AMHD |
2:50.86 |
2:51.70 |
0.84 |
| 3 |
Cribbs, Mila E (13)
|
AMPRA |
2:20.86 |
2:53.95 |
33.09 |
| 4 |
O'Connor, Brooklyn E (13)
|
AMRXA |
2:52.65 |
2:59.96 |
7.31 |
| 5 |
Kennedy, Delaney S (14)
|
AMANSC |
2:54.32 |
3:00.00 |
5.68 |
| 6 |
Medjimorec, Megan E (14)
|
AMPRA |
2:30.52 |
3:00.80 |
30.28 |
| 7 |
Hockinson, Zoe L (13)
|
AMANSC |
2:55.75 |
3:02.24 |
6.49 |
| 8 |
An, Emma K (13)
|
AMHD |
3:34.67 |
3:09.73 |
-24.94 |
| 9 |
Paul, Lillian L (13)
|
AMHD |
3:12.64 |
3:12.14 |
-0.50 |
| 10 |
Boronski, Alana C (13)
|
AMANSC |
18.78 |
3:14.35 |
175.57 |
| 11 |
Hanes, Kendall N (13)
|
AMANSC |
3:09.79 |
3:19.24 |
9.45 |
| 12 |
Watrobsky, Katelyn E (13)
|
AMHD |
3:43.98 |
3:32.31 |
-11.67 |
| 13 |
Burton, Salah M (14)
|
AMHD |
3:58.49 |
3:36.33 |
-22.16 |
|
Bugel, Talia M (14)
|
AMRXA |
2:25.48 |
DQ |
- |