| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Barnes, Olivia G (15)
|
GUBATS |
55.48 |
54.03 |
-1.45 |
| 2 |
Sisung, Lezli L (16)
|
GUBATS |
53.87 |
55.82 |
1.95 |
| 3 |
Ho, Lauren V (15)
|
GUBATS |
57.17 |
58.04 |
0.87 |
| 4 |
Torres, Celia G (18)
|
GUPACE |
59.03 |
59.08 |
0.05 |
| 5 |
Spano, Molly K (17)
|
GUUN-S |
57.46 |
59.27 |
1.81 |
| 6 |
Sarkari, Rukshana R (17)
|
GUUN-B |
58.98 |
59.69 |
0.71 |
| 7 |
Reitenour, Danielle N (15)
|
GUBLST |
57.34 |
1:00.15 |
2.81 |
| 8 |
Noonan, Miranda B (15)
|
GUBATS |
59.30 |
1:01.45 |
2.15 |
| 9 |
Norris, Grace L (15)
|
GUUN-B |
58.81 |
1:02.23 |
3.42 |
| 10 |
Rozelle, Laura M (15)
|
GUBATS |
1:01.90 |
1:03.83 |
1.93 |
| 11 |
Bracher, Caitlyn R (17)
|
GUBLST |
56.74 |
1:04.25 |
7.51 |
| 12 |
Nguyen, Tiffany A (16)
|
GUESCA |
1:05.97 |
1:05.00 |
-0.97 |
| 13 |
Willis, Alyssa A (15)
|
GUPLAT |
1:13.62 |
1:05.97 |
-7.65 |
| 14 |
Pierce, Morgan A (17)
|
GUUN-B |
59.87 |
1:06.48 |
6.61 |
| 15 |
Jensen-Morgan, Olive Y (16)
|
GUBATS |
1:05.55 |
1:06.73 |
1.18 |
| 16 |
Green, Emily E (15)
|
GUPACE |
1:03.16 |
1:06.78 |
3.62 |
| 17 |
Bourgeois-Solomon, Sovereign (15)
|
GUPACE |
1:04.07 |
1:06.92 |
2.85 |
| 18 |
Perez, Alydia M (16)
|
GUBLST |
1:02.17 |
1:06.98 |
4.81 |
| 19 |
Pumphrey, Loren N (15)
|
GUPLAT |
1:10.27 |
1:12.61 |
2.34 |
| 20 |
Bravo, Anabela M (15)
|
GUESCA |
|
1:20.30 |
- |
|
Spencer, Bo L (15)
|
GUUN-B |
57.39 |
NS |
- |