| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hughes, Catherine L (12)
|
PVAAC |
37.14 |
37.39 |
0.25 |
| 2 |
Sanchez, Lily A (12)
|
PVYORK |
44.03 |
44.09 |
0.06 |
| 3 |
Sims, Marisol W (11)
|
PVSNOW |
45.65 |
44.73 |
-0.92 |
| 4 |
Charters, Shannon A (11)
|
PVMACH |
48.97 |
45.52 |
-3.45 |
| 5 |
Rice, Ava E (12)
|
PVFXFX |
49.93 |
45.71 |
-4.22 |
| 6 |
Buckley, Kathlyn M (12)
|
PVSNOW |
45.89 |
49.06 |
3.17 |
| 7 |
Zuhoski, Olivia R (12)
|
PVMACH |
|
49.70 |
- |
| 8 |
Petty, Ava S (12)
|
PVFXFX |
49.71 |
50.46 |
0.75 |
| 9 |
Crapco-Wang, Caitlyn L (11)
|
PVMACH |
52.68 |
51.66 |
-1.02 |
| 10 |
Badrak, Audrey F (11)
|
PVMACH |
1:06.32 |
53.75 |
-12.57 |
| 11 |
Altenburg, Abigail W (12)
|
PVPM |
55.01 |
53.94 |
-1.07 |
|
Callahan, Grace H (11)
|
PVMACH |
45.70 |
NS |
- |
|
Cober, Makenzie F (12)
|
PVPM |
47.79 |
NS |
- |
|
Nguyen, Evi D (11)
|
PVPM |
56.01 |
NS |
- |