| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stukus, Aleena J (12)
|
PVMACH |
34.05 |
32.87 |
-1.18 |
| 2 |
Eziemefe, Adaora I (12)
|
PVMACH |
34.34 |
35.25 |
0.91 |
| 3 |
Schloth, Katherine I (11)
|
PVPM |
39.09 |
36.26 |
-2.83 |
| 4 |
Gieseman, Josephine C (12)
|
PVAAC |
|
36.30 |
- |
| 5 |
Sharrer, Katherine C (12)
|
PVYORK |
41.00 |
37.07 |
-3.93 |
| 6 |
Sturgill, Lydia K (11)
|
PVMACH |
40.52 |
37.78 |
-2.74 |
| 7 |
Read, Colleen E (12)
|
PVMACH |
36.58 |
38.34 |
1.76 |
| 8 |
Wilhite, Charlotte L (11)
|
PVMACH |
38.22 |
39.59 |
1.37 |
| 9 |
Petty, Ava S (12)
|
PVFXFX |
42.36 |
40.14 |
-2.22 |
| 10 |
Blake, Madeline C (12)
|
PVFXFX |
43.93 |
40.27 |
-3.66 |
| 11 |
Rossi, Helena D (11)
|
PVMAKO |
57.22 |
40.64 |
-16.58 |
| 12 |
Zuhoski, Olivia R (12)
|
PVMACH |
40.69 |
40.82 |
0.13 |
| 13 |
Kim, Allison S (11)
|
PVMAC |
41.43 |
42.26 |
0.83 |
|
Hartel, Olivia A (12)
|
PVAAC |
37.08 |
NS |
- |
|
Jackson, Isabelle L (12)
|
PVMACH |
39.49 |
NS |
- |
|
Tourbaf, Eliza J (12)
|
PVMACH |
35.10 |
NS |
- |