| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Buechler, Jordan N (14)
|
PVMACH |
|
2:38.32 |
- |
| 2 |
Martin, Natalie C (14)
|
PVAAC |
2:37.63 |
2:42.20 |
4.57 |
| 3 |
Schuman, Miranda A (13)
|
PVPM |
3:07.27 |
2:42.25 |
-25.02 |
| 4 |
Andrews, Emily (14)
|
PVAAC |
2:38.65 |
2:45.65 |
7.00 |
| 5 |
Church, Abby L (14)
|
PVMACH |
2:54.30 |
2:49.92 |
-4.38 |
| 6 |
Smith, Haley M (14)
|
PVMACH |
2:51.49 |
2:51.21 |
-0.28 |
| 7 |
Khorramshahgol, Salma N (13)
|
PVPM |
2:56.09 |
2:52.61 |
-3.48 |
| 8 |
Abogadie, Razelle B (14)
|
PVUN-M |
2:58.07 |
3:01.19 |
3.12 |
| 9 |
McCullough, Ainsley E (13)
|
PVMACH |
3:09.09 |
3:06.70 |
-2.39 |
| 10 |
Ren, Emily Y (14)
|
PVMACH |
3:03.58 |
3:07.64 |
4.06 |
| 11 |
Yoon, Sarah K (13)
|
PVPM |
3:07.50 |
3:12.91 |
5.41 |
| 12 |
McCullough, Teagan M (13)
|
PVMACH |
|
3:24.78 |
- |