| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Yu, Felix P (10)
|
PVMACH |
41.61 |
37.96 |
-3.65 |
| 2 |
Dockett, Landon S (10)
|
PVNCAP |
39.70 |
40.12 |
0.42 |
| 3 |
Edwards, Henry L (9)
|
PVMACH |
|
41.40 |
- |
| 4 |
Cherryholmes, Caleb J (10)
|
PVMACH |
|
42.43 |
- |
| 5 |
Nguyen, Declan L (10)
|
PVMACH |
46.27 |
42.83 |
-3.44 |
| 6 |
Tolar, Oliver M (10)
|
PVNCAP |
46.18 |
45.66 |
-0.52 |
| 7 |
Teufel, Pierce A (10)
|
PVMACH |
51.96 |
46.35 |
-5.61 |
| 8 |
Spinelli, Michael (10)
|
PVTOLL |
49.74 |
47.37 |
-2.37 |
| 9 |
Tutunaru, Mark-Robert (10)
|
PVNCAP |
49.02 |
48.26 |
-0.76 |
| 10 |
Zeng, Jalen (10)
|
PVTOLL |
|
48.51 |
- |
| 11 |
Beggan, Braxten K (9)
|
PVHACC |
49.35 |
48.53 |
-0.82 |
| 12 |
Tutkus, Tauras (10)
|
PVNCAP |
|
54.10 |
- |
| 13 |
Nguyen, Turner G (8)
|
PVMACH |
53.04 |
55.50 |
2.46 |
| 14 |
Kim, Kyle H (8)
|
PVTRA |
|
1:06.57 |
- |
|
McDonald, Ethan S (10)
|
PVNCAP |
46.93 |
NS |
- |
|
Liu, Ezekiel G (10)
|
PVNCAP |
50.00 |
NS |
- |
|
Wai, Anthony T (9)
|
PVNCAP |
46.02 |
NS |
- |
|
Berger, Colby J (9)
|
PVTOLL |
|
NS |
- |