| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hammond, Hailey G (8)
|
PVNCAP |
39.49 |
36.02 |
-3.47 |
| 2 |
Hastings, Clara W (8)
|
PVNCAP |
42.53 |
36.50 |
-6.03 |
| 3 |
Watts, Emma C (8)
|
PVFISH |
44.87 |
38.22 |
-6.65 |
| 4 |
Gilman, Heidi F (8)
|
PVHACC |
42.75 |
38.79 |
-3.96 |
| 5 |
Holland, Addison C (8)
|
PVNCAP |
40.21 |
39.89 |
-0.32 |
| 6 |
Marks, Stephanie N (8)
|
PVTIBU |
46.90 |
39.91 |
-6.99 |
| 7 |
Majeed-Hall, Aliyah M (8)
|
PVCSC |
47.05 |
39.95 |
-7.10 |
| 8 |
Yang, Julie (8)
|
PVNCAP |
41.08 |
40.93 |
-0.15 |
| 9 |
Mara, Litea R (8)
|
PVNCAP |
40.82 |
44.09 |
3.27 |
| 10 |
Kaul, Raleigh C (8)
|
PVAAC |
|
45.58 |
- |
| 11 |
Grenham, Kaitlyn R (8)
|
PVNCAP |
|
47.06 |
- |
| 12 |
Scoresby, Afton M (8)
|
PVNCAP |
|
47.55 |
- |
| 13 |
Liu, Rheechen (8)
|
PVHACC |
56.71 |
47.58 |
-9.13 |
| 14 |
de Souza, Paige A (8)
|
PVASA |
1:00.13 |
48.09 |
-12.04 |
| 15 |
Wratney, Elise C (8)
|
PVNCAP |
49.79 |
48.92 |
-0.87 |
| 16 |
Mulkey, Anne M (8)
|
PVNCAP |
|
50.76 |
- |
| 17 |
Klein, Hazel E (8)
|
PVNCAP |
50.15 |
51.54 |
1.39 |
| 18 |
Bridenbaugh, Nora E (8)
|
PVASA |
|
51.59 |
- |
| 19 |
Meka, Sanika (8)
|
PVNCAP |
57.94 |
52.23 |
-5.71 |
| 20 |
Hufnagel, Allison N (8)
|
PVNCAP |
1:04.50 |
52.90 |
-11.60 |
| 21 |
Wang, Julia M (8)
|
PVMACH |
|
53.55 |
- |
| 22 |
Melles, Delina M (8)
|
PVAAC |
1:00.22 |
55.43 |
-4.79 |
| 23 |
Chong, Sadie A (8)
|
PVTIBU |
1:08.33 |
58.08 |
-10.25 |
| 24 |
Hyun, Yesa A (8)
|
PVMACH |
|
59.21 |
- |
| 25 |
Wilson, Genevieve E (8)
|
PVMACH |
|
1:03.66 |
- |
| 25 |
Blumenthal, Madeline R (8)
|
PVCSC |
|
1:03.66 |
- |
| 27 |
Wade, Maya S (8)
|
PVFBST |
|
1:09.43 |
- |
| 28 |
Boyd, Kairo-Aicha F (8)
|
PVFBST |
|
1:09.60 |
- |
|
Pugsley, Jenna C (8)
|
PVNCAP |
1:00.64 |
NS |
- |