| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Harless, Madison E (16)
|
NTMTRO |
2:44.29 |
2:35.54 |
-8.75 |
| 2 |
Werth, Anna E (13)
|
NTMTRO |
2:32.47 |
2:35.95 |
3.48 |
| 3 |
Alvarado, Celeste A (15)
|
NTMAC |
2:41.68 |
2:36.85 |
-4.83 |
| 4 |
Titus, Leighton E (13)
|
NTMAC |
2:42.99 |
2:40.96 |
-2.03 |
| 5 |
Mills, Ashlyn M (13)
|
NTMAC |
2:41.75 |
2:43.22 |
1.47 |
| 6 |
Kruse, Kylie L (13)
|
NTMAC |
2:54.66 |
2:46.77 |
-7.89 |
| 7 |
Ferguson, Kaitlyn M (13)
|
NTMAC |
2:46.54 |
2:47.34 |
0.80 |
| 8 |
Clifford, Lexi A (16)
|
NTMTRO |
2:49.27 |
2:48.56 |
-0.71 |
| 9 |
Drori, Sarah (13)
|
NTMARS |
2:50.30 |
2:51.00 |
0.70 |
| 10 |
Brough, Regina C (13)
|
NTMAC |
3:07.63 |
2:52.86 |
-14.77 |
| 11 |
Carlson, Sophia L (13)
|
NTMARS |
2:51.58 |
2:54.21 |
2.63 |
| 12 |
Dietrich, Hannah K (13)
|
NTFAST |
2:51.71 |
2:57.37 |
5.66 |
| 13 |
Hughes-Shalom, Mila J (13)
|
NTMARS |
|
3:00.53 |
- |
| 14 |
Nsabimana, Gabrielle N (13)
|
NTMAC |
3:06.20 |
3:01.99 |
-4.21 |
| 15 |
Picinic, Giada G (14)
|
NTMARS |
|
3:07.39 |
- |
| 16 |
Rodriguez, Jocelyn G (16)
|
NTMAC |
|
3:17.15 |
- |
|
Kruzel, Peighton J (13)
|
NTMARS |
2:36.79 |
DQ |
- |
|
Luong, Ava T (13)
|
NTMTRO |
2:39.97 |
DQ |
- |
|
Hamlett, Lola E (13)
|
NTMARS |
3:11.24 |
NS |
- |
|
Lai, Megan M (14)
|
NTMAC |
|
NS |
- |