| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kim, Daniel H (9)
|
NTPSC |
41.86 |
39.12 |
-2.74 |
| 2 |
Kim, Hayoon (10)
|
NTPSC |
37.87 |
39.27 |
1.40 |
| 3 |
Case, James S (8)
|
NTESP |
41.57 |
39.73 |
-1.84 |
| 4 |
Dorsey, Luke H (8)
|
NTATAC |
42.35 |
40.40 |
-1.95 |
| 5 |
Ruhl, Noah D (9)
|
NTATAC |
40.47 |
42.07 |
1.60 |
| 6 |
Walker, Tyler A (10)
|
NTMAC |
43.42 |
44.09 |
0.67 |
| 7 |
Lamb, Landen D (10)
|
NTMTRO |
44.65 |
45.06 |
0.41 |
| 8 |
Youn, Ethan (10)
|
NTPSC |
45.15 |
45.28 |
0.13 |
| 9 |
Hackworth, Nathaniel D (10)
|
NTMTRO |
|
45.48 |
- |
| 10 |
Heimbuch, Dawson F (9)
|
NTPAC |
|
46.02 |
- |
| 11 |
Leverington, Risdon A (10)
|
NTO2 |
46.51 |
46.08 |
-0.43 |
| 12 |
Titus, Braiden A (10)
|
NTMAC |
46.69 |
46.56 |
-0.13 |
| 13 |
Gantchev, David A (10)
|
NTO2 |
48.40 |
47.26 |
-1.14 |
| 14 |
Lobdell, Alexander P (8)
|
NTO2 |
50.61 |
47.62 |
-2.99 |
| 15 |
Smith, Robert M (9)
|
NTMTRO |
50.44 |
48.45 |
-1.99 |
| 16 |
Pelusi, Scott T (8)
|
NTMAC |
|
48.95 |
- |
| 17 |
Pigneri, Michael A (8)
|
NTATAC |
48.55 |
49.66 |
1.11 |
| 18 |
Harris, Camden W (10)
|
NTMAC |
49.09 |
50.03 |
0.94 |
| 19 |
Petty, Rylen J (10)
|
NTMAC |
53.85 |
51.70 |
-2.15 |
| 20 |
Sureka, Sheel (7)
|
NTATAC |
53.94 |
53.45 |
-0.49 |
| 21 |
Kirk, Destin K (8)
|
NTMAC |
56.12 |
53.81 |
-2.31 |
| 22 |
Ray, Ronit (9)
|
NTO2 |
|
53.82 |
- |
| 23 |
Deo, Medhav (8)
|
NTO2 |
56.99 |
54.11 |
-2.88 |
| 24 |
Lugo, Sergio R (9)
|
NTMARS |
57.46 |
1:01.23 |
3.77 |
| 25 |
Gentry, Michael B (6)
|
NTATAC |
58.75 |
1:02.94 |
4.19 |
| 26 |
Beasley, Kaleb J (10)
|
NTMAC |
1:03.97 |
1:06.04 |
2.07 |
|
Prabhakara, Kushalgowda (9)
|
NTO2 |
|
DQ |
- |
|
Raghavan, Krish B (10)
|
NTMARS |
|
DQ |
- |
|
Fox, Dylan A (8)
|
NTESP |
47.14 |
NS |
- |
|
Bray, Travis H (8)
|
NTESP |
|
NS |
- |
|
Robertson, Lucas G (9)
|
NTUN-P |
51.16 |
NS |
- |