| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
O'Shaughnessy, Anne M (6)
|
PVFISH |
26.39 |
24.61 |
-1.78 |
| 2 |
Christofferson, Marie N (6)
|
PVMACH |
34.07 |
29.13 |
-4.94 |
| 3 |
Bishop, Alexandra (Lexie) M (6)
|
PVASA |
37.32 |
30.14 |
-7.18 |
| 4 |
Bendza, Corinne J (5)
|
PVMACH |
50.52 |
36.43 |
-14.09 |
| 5 |
Pezzimenti, Natalia M (5)
|
PVMACH |
|
37.78 |
- |
| 6 |
Hu, Kamilla K (6)
|
PVMACH |
|
42.80 |
- |
| 7 |
Nicholas, Chloe L (5)
|
PVHACC |
|
43.30 |
- |
|
Groves, Charlotte (4)
|
PVASA |
|
DQ |
- |
|
Walters, Lila M (6)
|
PVAAC |
|
DQ |
- |
|
Abrams, Sky Lynn H (6)
|
PVFBST |
|
DQ |
- |
|
Beckett, Isla K (6)
|
PVFBST |
|
DQ |
- |
|
Yiengst, Audrey V (5)
|
PVFBST |
|
DQ |
- |
|
Matthews, Aubree L (5)
|
PVNCAP |
|
DQ |
- |
|
Gault, Hazel S (5)
|
PVMACH |
|
NS |
- |