| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Adama, Zoe J (18)
|
NTMTRO |
28.26 |
27.58 |
-0.68 |
| 2 |
Kruzel, Peighton J (13)
|
NTMARS |
28.08 |
28.62 |
0.54 |
| 3 |
Luong, Ava T (13)
|
NTMTRO |
29.47 |
29.11 |
-0.36 |
| 4 |
Werth, Anna E (13)
|
NTMTRO |
28.68 |
29.24 |
0.56 |
| 5 |
Houdashell, Maya B (13)
|
NTMAC |
29.93 |
29.26 |
-0.67 |
| 6 |
Clifford, Lexi A (16)
|
NTMTRO |
30.52 |
29.89 |
-0.63 |
| 7 |
Titus, Leighton E (13)
|
NTMAC |
29.97 |
30.03 |
0.06 |
| 8 |
Menezes, Anisha (14)
|
NTMARS |
30.44 |
30.26 |
-0.18 |
| 9 |
Dangelmaier, Anja E (14)
|
NTMARS |
30.62 |
30.31 |
-0.31 |
| 10 |
Nsabimana, Gabrielle N (13)
|
NTMAC |
29.91 |
30.41 |
0.50 |
| 11 |
Hablo, Robi Amichie P (15)
|
NTMAC |
31.26 |
30.98 |
-0.28 |
| 12 |
DeYoung, Kathryn I (13)
|
NTMARS |
31.01 |
31.30 |
0.29 |
| 13 |
Constantine, Katherine E (13)
|
NTMARS |
31.83 |
31.34 |
-0.49 |
| 14 |
Hughes-Shalom, Mila J (13)
|
NTMARS |
34.79 |
31.86 |
-2.93 |
| 15 |
Drori, Sarah (13)
|
NTMARS |
32.46 |
33.13 |
0.67 |
| 16 |
Dietrich, Hannah K (13)
|
NTFAST |
32.33 |
34.17 |
1.84 |
| 17 |
Aspaas, Liv T (13)
|
NTMARS |
36.18 |
34.82 |
-1.36 |
| 18 |
Campos Canelas, Belen S (14)
|
NTMARS |
35.74 |
34.88 |
-0.86 |
| 19 |
Swinarsky, Caroline G (13)
|
NTMARS |
50.33 |
34.93 |
-15.40 |
| 20 |
Bonnette, Alyssa M (16)
|
NTMTRO |
40.90 |
39.22 |
-1.68 |
| 21 |
Schillo, Kathryn G (13)
|
NTMARS |
38.78 |
40.00 |
1.22 |
| 22 |
Morales, Maria D (13)
|
NTMARS |
|
46.33 |
- |
| 23 |
Hernandez Yanez, Dallane (14)
|
NTMARS |
|
49.94 |
- |
|
Grizzle, Samantha M (13)
|
NTMARS |
|
NS |
- |