| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Andruss, Lily G (11)
|
NTLAC |
31.97 |
29.64 |
-2.33 |
| 2 |
Cusack, Courtney M (11)
|
NTMTRO |
30.13 |
29.79 |
-0.34 |
| 3 |
Schneider, Natalie M (12)
|
NTLAC |
31.47 |
30.09 |
-1.38 |
| 4 |
Fisher, Alexis K (11)
|
NTDM |
30.62 |
30.37 |
-0.25 |
| 5 |
Lotrean, Carolina J (12)
|
NTNTN |
30.14 |
30.59 |
0.45 |
| 6 |
Yin, Julia (12)
|
NTMTRO |
31.27 |
30.71 |
-0.56 |
| 7 |
Johnson, Audrey E (12)
|
NTCOR |
31.12 |
30.76 |
-0.36 |
| 8 |
Feng, Katherine X (12)
|
NTLAC |
30.46 |
30.82 |
0.36 |
| 9 |
Estrada, Natalie (12)
|
NTDM |
30.88 |
31.10 |
0.22 |
| 10 |
Anderson, Stella V (11)
|
NTNTN |
31.03 |
31.24 |
0.21 |
| 11 |
Burau, Allison A (12)
|
NTDM |
30.86 |
31.36 |
0.50 |
| 12 |
Dewhirst, Isabella N (12)
|
NTESP |
31.05 |
31.43 |
0.38 |
| 13 |
Tucker, Caroline G (12)
|
NTCOR |
31.45 |
31.60 |
0.15 |
| 14 |
Stoklas, Catherine C (11)
|
NTNTN |
32.14 |
32.09 |
-0.05 |
| 15 |
Spencer, Macy L (12)
|
NTLAC |
32.63 |
32.66 |
0.03 |
| 16 |
Nguyen, Ellie T (11)
|
NTLAC |
31.75 |
32.74 |
0.99 |
| 17 |
Xi, Ziyuan (12)
|
NTLAC |
32.67 |
32.89 |
0.22 |
| 18 |
Masters, Cassidy K (11)
|
NTLAC |
34.51 |
33.28 |
-1.23 |
| 19 |
Meza, Rivkah (11)
|
NTTFA |
35.09 |
33.91 |
-1.18 |
| 20 |
Greer, McKennah K (11)
|
NTLAC |
36.86 |
35.20 |
-1.66 |