| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Morris, Ava M (12)
|
NCRACY |
46.94 |
38.74 |
-8.20 |
| 2 |
McCarty, Ava C (12)
|
SCYCSC |
41.86 |
38.95 |
-2.91 |
| 3 |
Sharpe, Sidda M (12)
|
SCAAYM |
|
40.24 |
- |
| 4 |
Burge, Jenna E (12)
|
SCNYST |
40.85 |
41.00 |
0.15 |
| 6 |
Medlin, Sadie J (12)
|
SCYCSC |
|
45.90 |
- |
| 7 |
Price, Cassidy A (12)
|
SCNYST |
43.19 |
46.68 |
3.49 |
| 8 |
Sun, Lily M (11)
|
NCWNCY |
|
46.79 |
- |
| 9 |
Weathers, Jillian W (11)
|
SCAAYM |
54.15 |
47.14 |
-7.01 |
| 11 |
Waters, Zoey L (11)
|
NCRACY |
|
51.86 |
- |
| 13 |
Jernigan, Jordyn M (11)
|
NCRACY |
1:03.22 |
56.32 |
-6.90 |
| 14 |
Fields, Sage M (11)
|
NCYSST |
52.56 |
57.14 |
4.58 |
| 17 |
Lineburger, Gracie B (11)
|
NCRACY |
|
1:05.72 |
- |
| 19 |
Chambers, Savannah M (11)
|
NCYSST |
|
1:21.73 |
- |