| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kilpatrick, Ava S (15)
|
NCWNCY |
25.23 |
25.56 |
0.33 |
| 3 |
Hernandez-Pena, Valeria (15)
|
NCYSST |
25.67 |
26.67 |
1.00 |
| 4 |
Parmenter, Katelyn L (14)
|
NCYSST |
25.94 |
26.99 |
1.05 |
| 5 |
Edwards, Emma K (15)
|
NCYSST |
26.11 |
27.21 |
1.10 |
| 6 |
Cunagin, Frances H (13)
|
NCWNCY |
28.02 |
27.92 |
-0.10 |
| 8 |
Gang, Camryn B (17)
|
NCWNCY |
26.88 |
28.40 |
1.52 |
| 9 |
Fields, Colby D (14)
|
NCYSST |
29.31 |
28.63 |
-0.68 |
| 10 |
Pitts, Emma W (13)
|
NCYSST |
28.15 |
28.89 |
0.74 |
| 11 |
Brake, Hannah M (16)
|
NCWNCY |
30.61 |
31.44 |
0.83 |
| 12 |
Thorpe, Katherine M (17)
|
NCYSST |
32.54 |
32.08 |
-0.46 |