| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kilpatrick, Ava S (14)
|
NCWNCY |
36.44 |
36.85 |
0.41 |
| 2 |
Hernandez-Pena, Valeria (14)
|
NCYSST |
39.78 |
38.47 |
-1.31 |
| 3 |
McCormack, Olivia A (14)
|
NCWNCY |
39.74 |
40.46 |
0.72 |
| 4 |
McGee, Isabella H (13)
|
NCRACY |
42.04 |
41.12 |
-0.92 |
| 5 |
Cattaneo, Courtney A (13)
|
SCYCSC |
39.42 |
41.66 |
2.24 |
| 6 |
Kaczynski, Abigail E (14)
|
SCYCSC |
|
42.21 |
- |
| 7 |
Seay, Aleah M (13)
|
NCWNCY |
|
42.72 |
- |
| 10 |
McCarty, Elizabeth M (13)
|
SCYCSC |
|
43.88 |
- |
| 11 |
Tippabhatla, Simran (13)
|
SCYCSC |
40.55 |
44.22 |
3.67 |
| 12 |
Jolly, Katherine E (13)
|
NCRACY |
|
45.82 |
- |
| 13 |
Weidner, Mia S (13)
|
NCWNCY |
57.87 |
50.88 |
-6.99 |
|
Shlien-Dellinger, Phoenix S (13)
|
NCWNCY |
41.06 |
NS |
- |