| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sutter, Olivia C (17)
|
NCWNCY |
32.41 |
33.62 |
1.21 |
| 2 |
Schlitt, Kayla M (17)
|
NCWNCY |
33.29 |
34.18 |
0.89 |
| 3 |
Taylor, Kayana R (15)
|
NCYSST |
37.13 |
36.03 |
-1.10 |
| 4 |
Turcanu, Nina C (17)
|
NCYSST |
34.53 |
36.12 |
1.59 |
| 5 |
Kilpatrick, Ava S (15)
|
NCWNCY |
36.44 |
36.13 |
-0.31 |
| 6 |
Hernandez-Pena, Valeria (15)
|
NCYSST |
38.17 |
36.96 |
-1.21 |
| 7 |
Edwards, Emma K (15)
|
NCYSST |
37.58 |
37.09 |
-0.49 |
| 8 |
McGuire, Grace M (16)
|
NCRACY |
41.63 |
40.06 |
-1.57 |
| 9 |
Kaczynski, Abigail E (15)
|
SCYCSC |
42.21 |
41.01 |
-1.20 |
| 10 |
Gang, Camryn B (16)
|
NCWNCY |
43.31 |
42.02 |
-1.29 |
| 12 |
Hart, Savannah R (15)
|
NCRACY |
40.78 |
42.49 |
1.71 |
| 14 |
Schreiber, Kaitlyn P (15)
|
SCYCSC |
43.35 |
44.62 |
1.27 |
| 15 |
Allen, Emily P (15)
|
SCNYST |
|
45.40 |
- |
| 16 |
Williams, Averi V (17)
|
NCWNCY |
|
46.88 |
- |
|
Reeder, Grace C (18)
|
NCWNCY |
31.84 |
NS |
- |