| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
He, Joshua S (17)
|
SIRSD |
56.51 |
56.52 |
0.01 |
| 2 |
Hildesheim, Ethan J (15)
|
SIRSD |
56.85 |
56.76 |
-0.09 |
| 3 |
Ranlett, Christopher (15)
|
SNDART |
58.06 |
58.64 |
0.58 |
| 4 |
Lewis, Tyler M (17)
|
OZPKWY |
|
59.29 |
- |
| 5 |
Huang, Roderick W (15)
|
PCSCSC |
59.27 |
59.43 |
0.16 |
| 6 |
Biscan, Jacob H (16)
|
MNEDI |
59.54 |
59.53 |
-0.01 |
| 7 |
Hayes, Aiden G (15)
|
OKSSC |
1:01.39 |
59.71 |
-1.68 |
| 8 |
Alessandria, Giorgio (18)
|
PCOAPB |
58.00 |
1:00.10 |
2.10 |
| 9 |
Cho, Amelia A (18)
|
SIRSD |
1:04.25 |
1:05.00 |
0.75 |
| 10 |
Li, Kaia J (15)
|
PCPASA |
1:07.20 |
1:05.01 |
-2.19 |
| 11 |
Kugler, Hattie M (16)
|
MNEDI |
1:04.99 |
1:05.47 |
0.48 |
| 12 |
Payne, Haily D (15)
|
IEVS |
1:07.08 |
1:05.99 |
-1.09 |
| 13 |
Anderson, Amanda N (17)
|
CAAZOT |
1:08.27 |
1:06.18 |
-2.09 |
| 14 |
Yeh, Phoebe Z (14)
|
CANOVA |
1:05.01 |
1:06.28 |
1.27 |
| 15 |
Duncan, Aurora R (16)
|
CAJCCW |
1:08.08 |
1:06.73 |
-1.35 |
| 16 |
Riederer, Muirenn F (15)
|
GUSSAN |
1:06.99 |
1:08.61 |
1.62 |