| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gao, Sophia (10)
|
ADCPP |
32.39 |
32.24 |
-0.15 |
| 2 |
Kao, Yirou (10)
|
ADJJSC |
38.79 |
36.88 |
-1.91 |
| 3 |
Cronlund, Miranda M (10)
|
ADDELM |
43.45 |
44.05 |
0.60 |
| 4 |
Bourassa, Annalise (10)
|
ADCPP |
|
44.94 |
- |
| 5 |
Singh, Sonia (10)
|
ADCPP |
|
46.86 |
- |
| 6 |
Perri, Gabriella M (9)
|
ADDELM |
44.38 |
47.43 |
3.05 |
| 7 |
Payamfar, Ranna (10)
|
ADALBS |
49.19 |
49.29 |
0.10 |
| 8 |
D'Amato, Victoria (9)
|
ADALBS |
|
55.11 |
- |
| 9 |
Zhang, Chloe H (9)
|
ADCPP |
|
58.28 |
- |
| 10 |
Martynyuk, Kajsa J (10)
|
ADBSPA |
|
1:01.19 |
- |
|
Eliea-Shields, Madelyn R (10)
|
ADDELM |
|
DQ |
- |
|
Holmes, Tess S (9)
|
NESNAP |
59.02 |
DQ |
- |
|
Appler, Madeline P (10)
|
ADNHA |
|
DQ |
- |
|
Rajulu, Maegna k (9)
|
ADDELM |
|
DQ |
- |
|
Rajulu, Hasni K (9)
|
ADDELM |
|
DQ |
- |
|
Fronheiser, Addison K (10)
|
ADDELM |
43.84 |
NS |
- |
|
Livak, Sadie M (9)
|
NESNAP |
49.12 |
NS |
- |