| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chen, Min-syun (11)
|
ADSARY |
37.32 |
37.18 |
-0.14 |
| 2 |
Altland, Elyse L (11)
|
ADBENN |
40.09 |
39.60 |
-0.49 |
| 3 |
Sorbello, Courtney H (11)
|
ADDELM |
40.51 |
39.81 |
-0.70 |
| 4 |
Erb, Elisabeth J (12)
|
ADSARY |
40.91 |
40.10 |
-0.81 |
| 5 |
Croke, Allison M (12)
|
ADADDI |
42.72 |
40.43 |
-2.29 |
| 6 |
Russom, Molly E (12)
|
ADDELM |
39.84 |
41.03 |
1.19 |
| 7 |
Savage, Lauren (11)
|
ADUN |
41.89 |
41.21 |
-0.68 |
| 8 |
Tandlmayer, Elizabeth A (11)
|
ADSSCY |
41.32 |
42.44 |
1.12 |
| 9 |
Baldwin, Elizabeth A (11)
|
ADDELM |
42.27 |
43.50 |
1.23 |
| 10 |
Clarke, Charlotte E (11)
|
ADDELM |
45.07 |
44.11 |
-0.96 |
| 11 |
Valerian, Caitlin G (12)
|
ADDELM |
43.34 |
44.19 |
0.85 |
| 12 |
Love, Rachel P (12)
|
ADSARY |
45.87 |
44.69 |
-1.18 |
| 13 |
Nolan, Celia H (12)
|
ADSARY |
47.19 |
45.29 |
-1.90 |
| 14 |
Skuza, Olivia (11)
|
ADDELM |
45.60 |
45.72 |
0.12 |
| 15 |
Barnaby-Wood, Arabelle M (11)
|
ADGRGF |
51.01 |
50.44 |
-0.57 |
| 16 |
Byrnes, Maeve C (11)
|
ADDELM |
50.80 |
50.54 |
-0.26 |
| 17 |
Harmon, Anna E (12)
|
ADBSPA |
56.50 |
53.58 |
-2.92 |
| 18 |
Dotel, Ferneidy R (12)
|
ADAMST |
56.30 |
55.54 |
-0.76 |
| 19 |
McManus, Aislinn M (11)
|
ADBENN |
|
1:13.62 |
- |
|
Coye, Isabella N (11)
|
ADSARY |
53.68 |
DQ |
- |
|
Girling, Chloe J (12)
|
ADSARY |
|
NS |
- |
|
Wall, Grace E (11)
|
ADSARY |
50.99 |
NS |
- |