| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mislow, John F (11)
|
MDEST |
30.70 |
29.12 |
-1.58 |
| 2 |
Barrett, Courtney E (11)
|
MDSPRC |
31.20 |
30.81 |
-0.39 |
| 3 |
Jenkins, Finn O (11)
|
MDCAA |
33.14 |
32.21 |
-0.93 |
| 4 |
Hogue, Tysen O (11)
|
MDRAC |
34.98 |
33.81 |
-1.17 |
| 5 |
Fuller, Mason A (11)
|
MDSPRC |
34.65 |
33.91 |
-0.74 |
| 6 |
Ileto, Matteo Gael T (11)
|
MDSPRC |
36.22 |
34.52 |
-1.70 |
| 7 |
Jing, Balwyn (11)
|
MDCAA |
35.56 |
34.90 |
-0.66 |
| 8 |
Glisson, Keith (11)
|
MDGBSA |
35.46 |
35.88 |
0.42 |
| 9 |
Kelley, Riordan E (11)
|
MDSPRC |
37.84 |
36.35 |
-1.49 |
| 10 |
Von Wachter, Caleb G (11)
|
MDUN |
38.44 |
36.71 |
-1.73 |
| 11 |
Gao, Daniel H (11)
|
MDCAA |
40.03 |
37.19 |
-2.84 |
| 12 |
Wan, Benjamin L (11)
|
MDCAA |
37.64 |
37.57 |
-0.07 |
| 13 |
Cosgrove, Ryan J (11)
|
MDMAC |
41.20 |
37.67 |
-3.53 |
| 14 |
Scheid, Nicholas K (11)
|
MDCAA |
38.84 |
37.80 |
-1.04 |
| 15 |
McNeill, Zachary L (11)
|
MDRAC |
39.94 |
38.09 |
-1.85 |
| 16 |
Burgoon, Ethan J (11)
|
MDCAA |
40.94 |
38.17 |
-2.77 |
| 17 |
Shi, Luke A (11)
|
MDCAA |
43.46 |
38.25 |
-5.21 |
| 18 |
Olenec, Callum A (11)
|
MDSPRC |
40.26 |
39.85 |
-0.41 |
| 19 |
Reed, Bennett C (11)
|
MDGBSA |
|
40.04 |
- |
| 20 |
Agboli, Ezra I (11)
|
MDCAA |
38.78 |
40.07 |
1.29 |
| 21 |
Shu, Bofeng C (11)
|
MDUN |
43.37 |
41.15 |
-2.22 |
| 22 |
Wong, Kieran J (11)
|
MDACA |
48.55 |
41.22 |
-7.33 |
| 23 |
Chiang, Lucas H (11)
|
MDCAA |
47.71 |
44.03 |
-3.68 |
| 24 |
Kowalczyk, Roman V (11)
|
MDCAA |
47.27 |
48.31 |
1.04 |
| 25 |
Walcott, Myles M (11)
|
MDCAA |
1:04.86 |
50.75 |
-14.11 |
|
Redmond, Matthew P (11)
|
MDUN |
1:20.93 |
DQ |
- |
|
Oh, Daniel J (11)
|
MDUN |
33.54 |
NS |
- |