| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Harper, Haily A (19)
|
MELRSC |
24.52 |
24.76 |
0.24 |
| 2 |
Park, Jaehee (18)
|
MELRSC |
24.87 |
24.88 |
0.01 |
| 3 |
Spelke, Cora A (17)
|
MELRSC |
26.30 |
25.46 |
-0.84 |
| 4 |
Jorgensen, Amanda N (18)
|
MELRSC |
25.77 |
25.92 |
0.15 |
| 5 |
Matthews, Kiley M (17)
|
MECMA |
26.51 |
25.97 |
-0.54 |
| 6 |
Farnham, Emma C (17)
|
MEMMD |
26.26 |
26.03 |
-0.23 |
| 7 |
Ericson, Alexandra W (16)
|
MECMA |
27.09 |
26.20 |
-0.89 |
| 8 |
Van Lonkhuyzen, Sarah G (16)
|
MEPBAY |
26.87 |
26.24 |
-0.63 |
| 9 |
Trundy, Elena D (15)
|
MELRSC |
27.24 |
26.27 |
-0.97 |
| 10 |
Matthews, Chloe C (15)
|
MECMA |
26.81 |
26.56 |
-0.25 |
| 11 |
Palmer, Sarah G (15)
|
MELRSC |
27.17 |
27.06 |
-0.11 |
| 12 |
Woodruff, Sadie M (17)
|
MEPBAY |
26.71 |
27.17 |
0.46 |
| 13 |
Carleton, Piper G (15)
|
MELRSC |
28.79 |
28.09 |
-0.70 |
| 14 |
Babikova, Issabel D (17)
|
MECMA |
26.81 |
28.53 |
1.72 |
|
McDonald, Juliana M (25)
|
MELRSC |
23.24 |
NS |
- |