| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Park, Jaehee (18)
|
MELRSC |
55.15 |
54.34 |
-0.81 |
| 2 |
Harper, Haily A (19)
|
MELRSC |
54.08 |
54.87 |
0.79 |
| 3 |
Farnham, Emma C (17)
|
MEMMD |
56.20 |
56.06 |
-0.14 |
| 4 |
Ericson, Alexandra W (16)
|
MECMA |
57.96 |
56.10 |
-1.86 |
| 5 |
Matthews, Kiley M (17)
|
MECMA |
57.00 |
56.74 |
-0.26 |
| 6 |
Matthews, Chloe C (15)
|
MECMA |
55.96 |
57.33 |
1.37 |
| 7 |
Jorgensen, Amanda N (18)
|
MELRSC |
56.38 |
57.39 |
1.01 |
| 8 |
Emmerson, Natalie B (15)
|
MELRSC |
59.34 |
58.87 |
-0.47 |
| 9 |
Trundy, Elena D (15)
|
MELRSC |
59.52 |
58.93 |
-0.59 |
| 10 |
Taylor, Grace E (16)
|
MECMA |
58.66 |
1:02.63 |
3.97 |
|
Van Lonkhuyzen, Sarah G (16)
|
MEPBAY |
56.63 |
DQ |
- |
|
McDonald, Juliana M (25)
|
MELRSC |
50.20 |
NS |
- |