| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jackson, Hunter C (10)
|
MRPATS |
29.26 |
29.21 |
-0.05 |
| 2 |
Holan, Steve (10)
|
MRPATS |
30.88 |
30.70 |
-0.18 |
| 3 |
Horgan, Edward L (10)
|
MRHVD |
32.73 |
31.86 |
-0.87 |
| 4 |
Sadchikov, Maximilian O (9)
|
MRUN-P |
33.12 |
32.58 |
-0.54 |
| 5 |
Vitale, Benjamin G (9)
|
MRPATS |
32.56 |
32.97 |
0.41 |
| 6 |
Anewalt, Christopher S (10)
|
MRHVD |
43.89 |
39.25 |
-4.64 |
| 7 |
Chun, Andrew (10)
|
MRHVD |
45.95 |
39.70 |
-6.25 |
| 8 |
Vermeulen, Lucas J (10)
|
MRPATS |
40.57 |
41.01 |
0.44 |
| 9 |
Beaudoin, Noah S (9)
|
MRHVD |
45.80 |
41.52 |
-4.28 |
| 10 |
Hayden, Killy J (10)
|
MRPATS |
48.14 |
47.38 |
-0.76 |
| 11 |
DeLuca, Christian (10)
|
MRPATS |
44.05 |
49.58 |
5.53 |
| 12 |
Carroll, Brandon L (10)
|
MRPATS |
1:00.65 |
54.31 |
-6.34 |