| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
van de Groenendaal, Akira (12)
|
ORTHSC |
32.23 |
31.55 |
-0.68 |
| 2 |
Kann, Connor A (11)
|
PCDACA |
34.44 |
34.95 |
0.51 |
| 3 |
Thistlethwaite, William I (12)
|
ORLOSC |
34.08 |
34.96 |
0.88 |
| 4 |
Zhang, Andrew (12)
|
PCDACA |
34.75 |
35.33 |
0.58 |
| 5 |
Patil, Arnav G (12)
|
PCDACA |
35.42 |
35.57 |
0.15 |
| 6 |
Acot, Rojel M (12)
|
PCDACA |
35.20 |
35.60 |
0.40 |
| 7 |
Stratton, Shaedon A (12)
|
SRBY |
34.27 |
36.63 |
2.36 |
| 8 |
Settle, Charles M (12)
|
ORTHSC |
36.68 |
37.67 |
0.99 |
| 9 |
Deitchler, Zachary B (11)
|
ORLOSC |
37.35 |
38.03 |
0.68 |
| 10 |
Yu, Kevin (11)
|
ORTHSC |
37.16 |
38.16 |
1.00 |
| 11 |
Kim, Minsong (11)
|
ORLOSC |
38.74 |
38.55 |
-0.19 |
| 12 |
Naim, Joshua A (12)
|
PCDACA |
40.08 |
39.83 |
-0.25 |
| 13 |
Zhang, Patrick Z (11)
|
ORLOSC |
42.58 |
41.11 |
-1.47 |
| 14 |
Xie, Justin J (12)
|
ORTHSC |
42.11 |
42.93 |
0.82 |
| 15 |
Kim, Tylor J (12)
|
ORLOSC |
43.32 |
43.31 |
-0.01 |
| 16 |
Freeman, Connor P (12)
|
ORTHSC |
49.22 |
46.11 |
-3.11 |
|
Ignatescu, Andrew T (12)
|
ORTHSC |
|
38.25 |
- |
|
Chapman, Jared I (12)
|
SRBY |
|
DQ |
- |