| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Devanagondi, Neeyati (14)
|
PCDACA |
25.32 |
25.20 |
-0.12 |
| 2 |
Lin, Helen (14)
|
ORTHSC |
24.85 |
25.24 |
0.39 |
| 3 |
Thomas, Sierra (14)
|
PCDACA |
24.88 |
25.35 |
0.47 |
| 4 |
Trainer, Hannah E (13)
|
ORTHSC |
25.31 |
25.37 |
0.06 |
| 5 |
Radle, Sarah W (14)
|
PCDACA |
25.28 |
25.56 |
0.28 |
| 6 |
Gingras, Elena S (14)
|
ORLOSC |
25.14 |
25.85 |
0.71 |
| 7 |
Maeda, Jessica Y (14)
|
ORTHSC |
25.67 |
26.08 |
0.41 |
| 8 |
Lindsay, Nakita L (14)
|
ORTHSC |
26.78 |
26.18 |
-0.60 |
| 9 |
Andersen, Olivia S (13)
|
ORLOSC |
26.59 |
26.24 |
-0.35 |
| 10 |
Bureau, Ella J (13)
|
SRBY |
25.75 |
26.40 |
0.65 |
| 11 |
Alharithi, Reem (14)
|
ORLOSC |
25.48 |
26.41 |
0.93 |
| 12 |
Chun, Audrey S (14)
|
ORLOSC |
26.78 |
26.53 |
-0.25 |
| 13 |
Katayama-Stall, Quinn T (14)
|
ORTHSC |
26.06 |
26.58 |
0.52 |
| 14 |
Pham, Minh-Anh (13)
|
PCDACA |
26.73 |
27.01 |
0.28 |
| 15 |
Andreani, Juliette E (14)
|
SRBY |
27.34 |
27.27 |
-0.07 |
| 16 |
Bureau, Aubrey A (13)
|
SRBY |
26.87 |
27.29 |
0.42 |
| 17 |
Barba, Rachel E (13)
|
ORLOSC |
27.43 |
28.35 |
0.92 |
| 18 |
Thompson, Audrey R (13)
|
SRUN |
29.30 |
28.86 |
-0.44 |
| 19 |
Caplener, Taylor J (13)
|
PCDACA |
27.58 |
29.08 |
1.50 |
| 20 |
Stevens, Kathryn J (13)
|
SRBY |
30.78 |
30.18 |
-0.60 |