| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sexton, Matthew S (13)
|
ORLOSC |
55.24 |
55.72 |
0.48 |
| 2 |
van de Groenendaal, Akira (14)
|
ORBLAC |
54.50 |
55.97 |
1.47 |
| 3 |
Bricca, Benjamin P (14)
|
PNSMAC |
55.82 |
56.08 |
0.26 |
| 4 |
Wu, Derek G (14)
|
ORLOSC |
59.54 |
58.64 |
-0.90 |
| 5 |
Kim, Tylor J (14)
|
ORLOSC |
59.41 |
58.76 |
-0.65 |
| 6 |
Ignatescu, Andrew T (14)
|
ORBLAC |
58.98 |
58.80 |
-0.18 |
| 7 |
Sanger, Frank J (14)
|
ORBLAC |
59.63 |
59.47 |
-0.16 |
| 8 |
Roedling, Liam A (13)
|
PCDACA |
59.17 |
59.61 |
0.44 |
| 9 |
Roller, Tyler J (13)
|
PCDACA |
1:00.00 |
1:00.85 |
0.85 |
| 10 |
Schreiber, Gabriel C (13)
|
ORLOSC |
1:00.45 |
1:00.92 |
0.47 |
| 11 |
Vu, Kyle N (14)
|
PNSMAC |
1:00.12 |
1:02.43 |
2.31 |
| 12 |
Matsumoto, Leona A (13)
|
PCDACA |
1:01.86 |
1:02.86 |
1.00 |
| 13 |
Scott, Benjamin R (13)
|
PNSMAC |
1:01.00 |
1:04.28 |
3.28 |
| 14 |
Hopkins, Gunner G (14)
|
PCDACA |
1:06.12 |
1:05.14 |
-0.98 |
| 15 |
Roy, Kartavya (14)
|
ORBLAC |
1:08.23 |
1:05.21 |
-3.02 |
| 16 |
Yuan, Albert N (13)
|
ORBLAC |
1:11.63 |
1:05.37 |
-6.26 |
|
Tran, Aaron H (14)
|
ORLOSC |
52.84 |
NS |
- |