| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Andrews, Catherine V (13)
|
PNSMAC |
56.02 |
53.84 |
-2.18 |
| 2 |
Gruner, Ava X (14)
|
PNOCA |
55.35 |
53.90 |
-1.45 |
| 3 |
Frangieh, Karly E (13)
|
PCDACA |
55.40 |
54.41 |
-0.99 |
| 4 |
Maher, Riley R (14)
|
ORTEAM |
55.12 |
54.98 |
-0.14 |
| 5 |
Schneider, Cassidy M (13)
|
ORLOSC |
56.90 |
55.37 |
-1.53 |
| 6 |
Larsen, Lauren G (14)
|
ORTEAM |
55.34 |
55.43 |
0.09 |
| 7 |
Pulaski, Sophia V (14)
|
ORLOSC |
56.46 |
55.59 |
-0.87 |
| 8 |
Schoenheit, Sephora A (13)
|
ORLOSC |
57.30 |
56.20 |
-1.10 |
| 9 |
Webster, Alastair B (13)
|
ORBLAC |
56.42 |
56.83 |
0.41 |
| 10 |
Golgotiu, Krista A (14)
|
OROCST |
56.18 |
56.84 |
0.66 |