| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Morini Bianzino, Tommaso (14)
|
PCDACA |
50.27 |
49.94 |
-0.33 |
| 2 |
Steiner, Alexander J (14)
|
ORHEAT |
50.83 |
50.05 |
-0.78 |
| 3 |
Corbeau, Angus R (14)
|
ORTHSC |
51.43 |
51.17 |
-0.26 |
| 4 |
Lei, Kevin (13)
|
ORTHSC |
52.76 |
52.37 |
-0.39 |
| 5 |
Richardson, Chase H (14)
|
ORLOSC |
53.54 |
52.56 |
-0.98 |
| 6 |
Gutzwiler, Daniel-Mathew A (14)
|
PNOCA |
53.23 |
53.01 |
-0.22 |
| 7 |
Yount, Karl J (14)
|
PNOCA |
53.91 |
53.26 |
-0.65 |
| 8 |
James, Conner A (14)
|
OROCST |
53.06 |
53.30 |
0.24 |
| 9 |
Tyshler, Daniel M (14)
|
PNOCA |
55.01 |
56.00 |
0.99 |
|
Purry, Elijah M (14)
|
ORHEAT |
52.62 |
DQ |
- |