| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Slevinski, Cameryn N (9)
|
NIEMAC |
46.11 |
46.44 |
0.33 |
| 2 |
Castellano, Riley E (10)
|
NIGVST |
52.85 |
49.66 |
-3.19 |
| 3 |
Nagel, Sienna R (9)
|
NIEMAC |
56.27 |
52.64 |
-3.63 |
| 4 |
Lanphier, Abigail R (10)
|
NIGVST |
58.26 |
54.87 |
-3.39 |
| 5 |
Oswald, Kaylee J (10)
|
NIGVST |
1:00.85 |
56.11 |
-4.74 |
| 6 |
Christensen, Kylee M (9)
|
NIGVST |
1:01.43 |
56.31 |
-5.12 |
| 7 |
Perkins, Reegan M (9)
|
NIGVST |
58.64 |
57.68 |
-0.96 |
| 8 |
Cooke, Makenna G (9)
|
NIGVST |
1:05.35 |
1:01.72 |
-3.63 |
| 9 |
White, Carissa M (10)
|
NIGVST |
1:06.27 |
1:05.54 |
-0.73 |
| 10 |
O'Connor, Talia (9)
|
NIGVST |
1:00.81 |
1:08.22 |
7.41 |
| 11 |
Shutt, Halle E (9)
|
NIGVST |
1:14.15 |
1:14.70 |
0.55 |
| 12 |
Bidzerkowny, Samantha R (10)
|
NIGVST |
1:20.19 |
1:16.50 |
-3.69 |
|
Marciano, Mackenzie L (10)
|
NIGVST |
1:13.03 |
NS |
- |