| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mattison, Morgan E (10)
|
NIGVST |
40.03 |
40.45 |
0.42 |
| 2 |
Baker, Chloe R (10)
|
NIEMAC |
36.93 |
42.21 |
5.28 |
| 3 |
Perkins, Reegan M (9)
|
NIGVST |
42.24 |
43.23 |
0.99 |
| 4 |
Castellano, Riley E (10)
|
NIGVST |
41.76 |
44.01 |
2.25 |
| 5 |
Mattison, Sarah N (9)
|
NIGVST |
49.18 |
44.23 |
-4.95 |
| 6 |
Lanphier, Abigail R (10)
|
NIGVST |
49.18 |
45.32 |
-3.86 |
| 7 |
Nagel, Sienna R (9)
|
NIEMAC |
58.08 |
48.89 |
-9.19 |
| 8 |
Oswald, Kaylee J (10)
|
NIGVST |
53.55 |
49.63 |
-3.92 |
| 9 |
Outman, Jasmine D (10)
|
NIGVST |
47.47 |
50.40 |
2.93 |
| 10 |
Ireland, Alexis M (10)
|
NIGVST |
47.25 |
50.88 |
3.63 |
| 11 |
Bidzerkowny, Samantha R (10)
|
NIGVST |
54.22 |
51.48 |
-2.74 |
| 12 |
Cooke, Makenna G (9)
|
NIGVST |
53.42 |
54.62 |
1.20 |
| 13 |
Shutt, Halle E (9)
|
NIGVST |
59.32 |
54.99 |
-4.33 |
| 14 |
White, Carissa M (10)
|
NIGVST |
1:04.86 |
1:06.86 |
2.00 |
| 15 |
O'Connor, Talia (9)
|
NIGVST |
55.96 |
1:07.80 |
11.84 |
|
Marciano, Mackenzie L (10)
|
NIGVST |
1:00.45 |
NS |
- |