| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Snayczuk, Madison J (14)
|
NIAAAC |
26.64 |
27.57 |
0.93 |
| 2 |
Pecorella, Brooke E (13)
|
NIEMAC |
27.84 |
29.32 |
1.48 |
| 3 |
McCutcheon, Chloe M (13)
|
NIAAAC |
28.46 |
29.69 |
1.23 |
| 4 |
Cadle, Mattison I (13)
|
NIPVSC |
28.96 |
29.85 |
0.89 |
| 5 |
Harrington, Porsha M (13)
|
NIAAAC |
30.03 |
30.18 |
0.15 |
| 6 |
Keesler, Kaylynn N (13)
|
NIEMAC |
30.57 |
30.94 |
0.37 |
| 7 |
Mikolajczyk, Emma R (14)
|
NIEMAC |
30.94 |
31.15 |
0.21 |
| 8 |
Slavinski, Anna C (13)
|
NIFKVL |
30.12 |
31.24 |
1.12 |
| 9 |
Ground, Heather A (14)
|
NISAAC |
32.07 |
31.32 |
-0.75 |
| 10 |
Coble, Abbigail M (13)
|
NIGVST |
31.08 |
31.82 |
0.74 |
| 11 |
LaBenne, Ellie B (13)
|
NIGVST |
33.65 |
33.80 |
0.15 |
| 12 |
Castellano, Madison A (14)
|
NIGVST |
35.14 |
35.23 |
0.09 |
| 13 |
Oswald, Stephanie R (14)
|
NIGVST |
36.25 |
35.26 |
-0.99 |
| 14 |
Kerr, Aurora A (14)
|
NIPVSC |
34.55 |
35.84 |
1.29 |
| 15 |
Wetherby, Breanne A (13)
|
NIEMAC |
46.84 |
47.63 |
0.79 |
|
Butler, Kennedy L (13)
|
NIGVST |
30.30 |
NS |
- |
|
Hammond, Taylor M (13)
|
NIEMAC |
37.58 |
NS |
- |