| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dolan, Reilly D (11)
|
MAUMLY |
41.10 |
41.17 |
0.07 |
| 2 |
Nowoswiat, Jena M (12)
|
MAUMLY |
40.99 |
42.56 |
1.57 |
| 3 |
Ladd, Caelan (12)
|
MAWCAY |
43.48 |
43.62 |
0.14 |
| 4 |
Hammerschmidt, Anna C (11)
|
MAKAY |
48.49 |
43.87 |
-4.62 |
| 5 |
Fennimore, Chloe L (11)
|
MAPAY |
44.33 |
44.44 |
0.11 |
| 6 |
Grant, Katherine G (12)
|
MABRY |
46.31 |
44.60 |
-1.71 |
| 7 |
Hailu, Yohanna (12)
|
MAWEST |
44.27 |
44.61 |
0.34 |
| 8 |
Perrin, Lily A (12)
|
MAKAY |
44.94 |
44.73 |
-0.21 |
| 9 |
Semisch, Skyler G (12)
|
MAPAY |
44.99 |
45.05 |
0.06 |
| 10 |
McCullough, Saryn R (12)
|
MAWEST |
46.46 |
46.24 |
-0.22 |
| 11 |
Liu, Jasmine L (11)
|
MAKAY |
52.14 |
46.68 |
-5.46 |
| 12 |
Brandberg, Essa L (11)
|
MAUMLY |
48.86 |
48.30 |
-0.56 |
| 13 |
Whitton, Sloane E (12)
|
MAWEST |
48.57 |
48.31 |
-0.26 |
| 14 |
Westerman, Amanda R (11)
|
MAPAY |
48.80 |
49.20 |
0.40 |
| 15 |
Basu, Anika (12)
|
MAUMLY |
55.83 |
52.15 |
-3.68 |
| 16 |
Van Patten, Madelynn (11)
|
MDYCM |
52.86 |
53.88 |
1.02 |