| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Opitz, Lauren E (12)
|
AMPEAQ |
3:01.99 |
2:56.30 |
-5.69 |
| 2 |
Edner, Callahan E (12)
|
AMBPR |
3:09.00 |
3:03.02 |
-5.98 |
| 3 |
Konvolinka, Livia A (11)
|
AMSVSC |
3:08.65 |
3:13.62 |
4.97 |
| 4 |
Buettner, Aubrey A (12)
|
AMSHAQ |
3:36.32 |
3:16.75 |
-19.57 |
| 5 |
Allen, Julina M (12)
|
AMWAAC |
3:23.88 |
3:24.61 |
0.73 |
| 6 |
Clodfelter, Claire L (11)
|
AMPEAQ |
3:45.13 |
3:25.19 |
-19.94 |
| 7 |
Greco, Claire A (12)
|
AMBPR |
3:59.99 |
3:25.33 |
-34.66 |
| 8 |
Sirianni, Isabella S (12)
|
AMPEAQ |
3:31.08 |
3:33.27 |
2.19 |
| 9 |
Barone, Lyannie G (12)
|
AMSHAQ |
3:36.37 |
3:38.46 |
2.09 |
| 10 |
Schoeffel, Alyssa M (12)
|
AMSVSC |
4:08.92 |
3:59.38 |
-9.54 |
| 11 |
Perine, Abriella A (11)
|
AMSVSC |
4:03.05 |
4:03.66 |
0.61 |
|
Zorey, Addison J (12)
|
AMWAAC |
3:40.00 |
DQ |
- |