| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Slack, Victoria R (13)
|
NICVSC |
30.11 |
29.70 |
-0.41 |
| 2 |
Rice, Lindsey A (13)
|
NICAGO |
30.37 |
30.29 |
-0.08 |
| 3 |
Neville, Victoria M (14)
|
NICAGO |
30.65 |
30.30 |
-0.35 |
| 4 |
Cervoni, Peighton R (14)
|
NIGGST |
32.73 |
31.68 |
-1.05 |
| 5 |
Jarvis, Amy C (13)
|
NIWAAC |
32.31 |
33.09 |
0.78 |
| 6 |
Dorak, Mary E (13)
|
NICAGO |
36.66 |
35.83 |
-0.83 |
| 7 |
Kopatz, Brooke H (13)
|
NIWAAC |
38.09 |
35.99 |
-2.10 |
| 8 |
Whitley, Maggie J (13)
|
NIWAAC |
32.94 |
36.54 |
3.60 |
| 9 |
Baek, Ashley H (13)
|
NIUNAC |
38.04 |
37.04 |
-1.00 |
| 10 |
Trumino, Emma L (14)
|
NICVSC |
|
37.80 |
- |
| 11 |
Williams, Samantha L (13)
|
NICAGO |
|
52.25 |
- |
|
Betts, Lillian E (13)
|
NIGGST |
1:07.68 |
NS |
- |
|
Guilfoyle, Shannon G (14)
|
NIUNAC |
46.74 |
NS |
- |