| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Potenziano, Taylor (17)
|
NICVSC |
29.38 |
28.97 |
-0.41 |
| 2 |
Kittle, Kayla A (16)
|
NIWAAC |
31.19 |
30.04 |
-1.15 |
| 3 |
Harford, Starr V (16)
|
NIWAAC |
31.77 |
30.68 |
-1.09 |
| 4 |
Bishop, Selkie G (18)
|
NIWAAC |
31.43 |
30.99 |
-0.44 |
| 5 |
Grunder, Hannah (17)
|
NICAGO |
30.96 |
31.82 |
0.86 |
| 6 |
Gorsline, Kendra L (17)
|
NIWAAC |
35.07 |
32.75 |
-2.32 |
| 7 |
Christensen, Taylor J (18)
|
ADUN-S |
29.02 |
32.88 |
3.86 |
| 7 |
Madigan, Kelcey H (18)
|
NIWAAC |
39.90 |
32.88 |
-7.02 |
| 9 |
Shay, Jillian M (16)
|
NIWAAC |
35.12 |
34.01 |
-1.11 |
| 10 |
Bard, Christina A (16)
|
NIWAAC |
|
34.65 |
- |
| 11 |
Huddleston, Chenelle M (17)
|
NIWAAC |
40.83 |
36.09 |
-4.74 |
| 12 |
Trumino, Emma L (15)
|
NICVSC |
37.80 |
36.64 |
-1.16 |
|
Neville, Victoria M (15)
|
NICAGO |
30.30 |
NS |
- |