| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mech, Olivia G (7)
|
ADSIDN |
34.64 |
24.32 |
-10.32 |
| 2 |
Ioannisci, Gianna M (8)
|
NIUNAC |
26.86 |
24.48 |
-2.38 |
| 3 |
Klemann, Aubrey s (8)
|
NIGGST |
27.10 |
24.92 |
-2.18 |
| 4 |
Neubert, Erin A (7)
|
ADSIDN |
28.15 |
25.60 |
-2.55 |
| 5 |
Chandler, Nora M (8)
|
NICVSC |
26.39 |
25.75 |
-0.64 |
| 6 |
Tyler, Natalie L (8)
|
NICVSC |
26.42 |
26.15 |
-0.27 |
| 7 |
Hubenthal, Mackenzie R (7)
|
NICVSC |
35.08 |
28.01 |
-7.07 |
| 8 |
Bucci, Giavanna (8)
|
NICVSC |
28.31 |
28.19 |
-0.12 |
| 9 |
Spicher, Lillian R (8)
|
NIGGST |
26.96 |
28.88 |
1.92 |
| 10 |
Spencer, Danielle H (6)
|
NIWAAC |
31.91 |
30.55 |
-1.36 |
| 11 |
Baldwin, Lily M (6)
|
NICVSC |
35.19 |
31.92 |
-3.27 |
| 12 |
Murphy, Mackenzie E (6)
|
NICVSC |
33.89 |
32.89 |
-1.00 |
| 13 |
Fitzwater, Elizabeth O (8)
|
NIGGST |
36.27 |
36.84 |
0.57 |
|
Mitchell, Hope J (8)
|
NIGGST |
23.49 |
NS |
- |
|
Stank, Amelia D (6)
|
NIUNAC |
24.32 |
NS |
- |