| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McWhorter, Peyton E (15)
|
SCMBR |
25.94 |
25.76 |
-0.18 |
| 2 |
Pottle, Kate B (17)
|
NCCFAC |
25.66 |
26.11 |
0.45 |
| 3 |
Bazinet, Laurie Anne (15)
|
NCWOW |
25.06 |
26.13 |
1.07 |
| 4 |
Phillips, Paige M (15)
|
NCWOW |
26.20 |
26.53 |
0.33 |
| 5 |
Black, Kathryn E (17)
|
NCWOW |
25.54 |
26.60 |
1.06 |
| 6 |
White, McKenzie E (17)
|
NCBAC |
25.31 |
26.79 |
1.48 |
| 7 |
Hyldahl, Aubrey E (16)
|
NCWOW |
27.00 |
26.99 |
-0.01 |
| 8 |
Marshall, Lauren D (17)
|
NCWOW |
27.67 |
27.04 |
-0.63 |
| 9 |
Teeters, Hannah L (17)
|
NCCFAC |
25.58 |
27.05 |
1.47 |
| 10 |
Denton, Emma G (16)
|
NCGFSC |
26.42 |
27.10 |
0.68 |
| 11 |
Ortiz, Elaine G (16)
|
NCGFSC |
27.09 |
27.16 |
0.07 |
| 12 |
Hoover, Madison M (15)
|
NCWOW |
25.71 |
27.40 |
1.69 |
| 13 |
Hannum, Ellie G (15)
|
NCWOW |
27.47 |
27.43 |
-0.04 |
| 14 |
Marinoff, Victoria (17)
|
SCCAC |
26.99 |
27.51 |
0.52 |
| 15 |
Popella, Delaney L (16)
|
NCCFAC |
25.19 |
27.60 |
2.41 |
| 16 |
Keller, Breanna D (17)
|
NCBAC |
28.65 |
27.75 |
-0.90 |
| 17 |
Whaley, Anna S (17)
|
NCCFAC |
27.43 |
27.79 |
0.36 |
| 18 |
McGlade, Kylie S (15)
|
NCGFSC |
27.41 |
28.49 |
1.08 |
| 19 |
Savard, Jocelyn I (16)
|
NCCFAC |
27.84 |
28.66 |
0.82 |
| 20 |
Ball, Emily A (15)
|
NCCFAC |
27.81 |
28.93 |
1.12 |
| 21 |
Earl, Ashley E (16)
|
NCGFSC |
28.71 |
29.48 |
0.77 |
| 22 |
Palmquist, Elizabeth M (18)
|
NCBAC |
29.31 |
29.57 |
0.26 |
| 23 |
Bunton, Raven K (16)
|
SCCAC |
29.03 |
29.71 |
0.68 |
| 24 |
Riffel, Lauren M (16)
|
NCBAC |
30.76 |
30.49 |
-0.27 |