| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Graham, Cameron A (11)
|
OHUSA |
1:27.16 |
1:25.16 |
-2.00 |
| 2 |
Mahan, Alayna R (12)
|
OHBGSC |
1:32.70 |
1:30.59 |
-2.11 |
| 3 |
Ehret, Audrey E (12)
|
OHDCST |
1:31.22 |
1:31.47 |
0.25 |
| 4 |
Moore, Alexis A (12)
|
OHUSA |
1:32.40 |
1:31.90 |
-0.50 |
| 5 |
Heath, Riley A (12)
|
OHGTAC |
1:33.69 |
1:33.53 |
-0.16 |
| 6 |
Majumdar, Ryka (12)
|
OHWOR |
1:34.77 |
1:34.34 |
-0.43 |
| 7 |
Johanssen, Katelyn C (12)
|
OHUSA |
1:39.09 |
1:36.54 |
-2.55 |
| 8 |
Seelandt, Ella M (12)
|
OHBKYS |
1:37.96 |
1:37.31 |
-0.65 |
| 8 |
Randolph, Jillian J (11)
|
OHDCST |
1:36.48 |
1:37.31 |
0.83 |
| 10 |
Martin, Caroline A (12)
|
OHDCST |
1:41.29 |
1:38.51 |
-2.78 |
| 11 |
Pyles, Madison E (12)
|
OHUSA |
1:37.20 |
1:38.53 |
1.33 |
| 12 |
Claggett, Brookelyn O (12)
|
OHBKYS |
1:38.94 |
1:39.23 |
0.29 |
| 13 |
Baxter, Charlotte L (11)
|
OHFMST |
1:41.34 |
1:42.26 |
0.92 |
| 14 |
Nixon, Natalie P (11)
|
OHGTAC |
1:48.08 |
1:47.53 |
-0.55 |
| 15 |
Wojciechowski, Harper M (11)
|
OHFMST |
1:52.94 |
1:48.51 |
-4.43 |
| 16 |
Avram, Sofia C (12)
|
OHGTAC |
1:30.62 |
1:49.12 |
18.50 |
| 17 |
Wittmer, Avery L (11)
|
OHFMST |
1:51.99 |
1:49.37 |
-2.62 |
| 18 |
Drerup, Claire E (12)
|
OHGTAC |
1:49.96 |
1:56.44 |
6.48 |
| 19 |
Diller, Kylee J (11)
|
OHFMST |
2:02.02 |
1:59.87 |
-2.15 |
| 20 |
Kline, Camryn E (11)
|
OHBKYS |
2:08.91 |
2:05.37 |
-3.54 |