| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Buff, Mia A (13)
|
OHGTAC |
33.37 |
29.54 |
-3.83 |
| 2 |
Freeworth, Kendall L (14)
|
OHSYL |
32.09 |
30.86 |
-1.23 |
| 3 |
Thomas, Addison P (14)
|
OHDCST |
34.17 |
30.90 |
-3.27 |
| 4 |
Arnett, Macie K (13)
|
OHUSA |
31.69 |
32.10 |
0.41 |
| 5 |
Richner, Madelyn L (13)
|
OHWOR |
35.56 |
33.13 |
-2.43 |
| 6 |
Bower, Molly L (14)
|
OHWOR |
33.00 |
34.09 |
1.09 |
| 7 |
Crandell, Cameron M (13)
|
OHGTAC |
40.08 |
34.16 |
-5.92 |
| 8 |
Kincaid, Madison E (14)
|
OHUSA |
|
34.50 |
- |
| 9 |
Aung, Suri L (13)
|
OHLTPY |
38.26 |
34.88 |
-3.38 |
| 10 |
Sharp, Avery J (13)
|
OHFMST |
33.36 |
34.98 |
1.62 |
| 11 |
Ivancso, Katherine M (14)
|
OHSYL |
38.58 |
35.09 |
-3.49 |
| 12 |
Randolph, Kylie A (14)
|
OHFMST |
33.47 |
35.28 |
1.81 |
| 13 |
Nixon, Audrey G (13)
|
OHGTAC |
44.09 |
35.58 |
-8.51 |
| 14 |
Eiferman, Julia L (14)
|
OHUSA |
39.09 |
35.88 |
-3.21 |
| 15 |
Diller, Aubrey L (14)
|
OHFMST |
36.35 |
37.11 |
0.76 |
| 16 |
Schmidt, Cienna G (13)
|
OHKCST |
38.76 |
37.13 |
-1.63 |
| 17 |
Schneider, Lily A (13)
|
OHUSA |
52.13 |
37.67 |
-14.46 |