| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Julian, Wyatt A (14)
|
OHUSA |
38.69 |
29.57 |
-9.12 |
| 2 |
Vertikoff, Michael A (14)
|
OHUSA |
34.18 |
29.82 |
-4.36 |
| 3 |
Freeworth, Keegan C (14)
|
OHSYL |
34.49 |
29.93 |
-4.56 |
| 4 |
King, Bradley N (13)
|
OHUSA |
34.65 |
30.06 |
-4.59 |
| 5 |
Montgomery, Cole J (13)
|
OHWOR |
31.18 |
31.09 |
-0.09 |
| 6 |
Sanchez, Brady M (14)
|
OHSYL |
33.87 |
31.21 |
-2.66 |
| 7 |
Moore, Caleb M (13)
|
OHUSA |
|
34.10 |
- |
| 8 |
Peters, Cole E (13)
|
OHGTAC |
35.51 |
34.84 |
-0.67 |
| 9 |
Wirantana, Tobias K (14)
|
OHUSA |
43.12 |
35.19 |
-7.93 |
| 10 |
Bliss, Nathan P (14)
|
OHUSA |
|
35.25 |
- |
| 12 |
Poland, Nathaniel P (13)
|
OHFMST |
44.16 |
39.30 |
-4.86 |
| 13 |
Sanchez, Ian J (13)
|
OHSYL |
46.10 |
43.08 |
-3.02 |
|
Rodriguez, Brendon A (14)
|
OHGCST |
35.99 |
NS |
- |