| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carter, Mackenzie M (16)
|
OHGCST |
30.66 |
30.11 |
-0.55 |
| 2 |
Laycock, Charlotte I (16)
|
OHUSA |
30.17 |
30.43 |
0.26 |
| 3 |
Spangler, Bethany D (15)
|
OHEP |
33.21 |
30.90 |
-2.31 |
| 4 |
Campbell, Nanami (16)
|
OHWOR |
30.68 |
31.08 |
0.40 |
| 5 |
Schiele, Emily N (15)
|
OHEP |
28.16 |
31.94 |
3.78 |
| 6 |
Hofmeister, Morgan R (16)
|
OHUSA |
38.27 |
32.10 |
-6.17 |
| 7 |
Randolph, Elise R (15)
|
OHFMST |
31.06 |
32.32 |
1.26 |
| 8 |
Price, Teagan M (16)
|
OHUSA |
32.08 |
32.67 |
0.59 |
| 9 |
Tennant, Mia E (15)
|
OHSYL |
37.38 |
34.71 |
-2.67 |
| 10 |
Freeman, Sylvia R (15)
|
OHFMST |
34.93 |
36.14 |
1.21 |
| 11 |
Neese, Chayse J (16)
|
OHGCST |
39.35 |
36.22 |
-3.13 |